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pegfp c1  (TaKaRa)


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    Structured Review

    TaKaRa pegfp c1
    Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1/pmCherry-C1+Vector/bio_rxiv__64898__2026__03__03__709279-248-18-23
    Average 96 stars, based on 3286 article reviews
    pegfp c1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Membrane Curvature Activates Src kinase and Promotes Metastatic Cancer Cell Survival
    Article Snippet: .. These DNA fragments were used to replace the EGFP in pEGFP-N1 for mammalian expression, or inserted into pEGFP-N1, pEGFP-C1, pmCherry-N1 or pmCherry-C1 vector (Clontech) for the expression of GFP- or RFP-tagged constructs. ..

    Plasmid Preparation:

    Article Title: Membrane Curvature Activates Src kinase and Promotes Metastatic Cancer Cell Survival
    Article Snippet: .. These DNA fragments were used to replace the EGFP in pEGFP-N1 for mammalian expression, or inserted into pEGFP-N1, pEGFP-C1, pmCherry-N1 or pmCherry-C1 vector (Clontech) for the expression of GFP- or RFP-tagged constructs. ..

    Article Title: Bacteria based protein delivery
    Article Snippet: .. Further at the 3′ end of YopE1-138 the following cleavage sites were added: XbaI-XhoI-BstBI-(HindIII) (FIG. 3 B). pBad_Si1 is equal to pBad_Si2 but encodes EGFP amplified from pEGFP-C1 (Clontech) in the NcoI/BglII site under the Arabinose inducible promoter. pT3P-715 (FIG. 4) is a fully synthetic plasmid (de-novo synthesized vector) with similar characteristics to pSi_2, while the corresponding AraC coding region has been deleted, and the Ampicillin resistance gene (plus 70 bp upstream) is replaced by a chlorampenicol resistance gene with 200 bp upstream region. ..

    Construct:

    Article Title: Membrane Curvature Activates Src kinase and Promotes Metastatic Cancer Cell Survival
    Article Snippet: .. These DNA fragments were used to replace the EGFP in pEGFP-N1 for mammalian expression, or inserted into pEGFP-N1, pEGFP-C1, pmCherry-N1 or pmCherry-C1 vector (Clontech) for the expression of GFP- or RFP-tagged constructs. ..

    Amplification:

    Article Title: Bacteria based protein delivery
    Article Snippet: .. Further at the 3′ end of YopE1-138 the following cleavage sites were added: XbaI-XhoI-BstBI-(HindIII). pBad_Si1 is equal to pBad_Si2 but encodes EGFP amplified from pEGFP-C1 (Clontech) in the NcoI/BglII site under the Arabinose inducible promoter. ..

    Article Title: Bacteria based protein delivery
    Article Snippet: .. Further at the 3′ end of YopE1-138 the following cleavage sites were added: XbaI-XhoI-BstBI-(HindIII) (FIG. 3 B). pBad_Si1 is equal to pBad_Si2 but encodes EGFP amplified from pEGFP-C1 (Clontech) in the NcoI/BglII site under the Arabinose inducible promoter. pT3P-715 (FIG. 4) is a fully synthetic plasmid (de-novo synthesized vector) with similar characteristics to pSi_2, while the corresponding AraC coding region has been deleted, and the Ampicillin resistance gene (plus 70 bp upstream) is replaced by a chlorampenicol resistance gene with 200 bp upstream region. ..

    Synthesized:

    Article Title: Bacteria based protein delivery
    Article Snippet: .. Further at the 3′ end of YopE1-138 the following cleavage sites were added: XbaI-XhoI-BstBI-(HindIII) (FIG. 3 B). pBad_Si1 is equal to pBad_Si2 but encodes EGFP amplified from pEGFP-C1 (Clontech) in the NcoI/BglII site under the Arabinose inducible promoter. pT3P-715 (FIG. 4) is a fully synthetic plasmid (de-novo synthesized vector) with similar characteristics to pSi_2, while the corresponding AraC coding region has been deleted, and the Ampicillin resistance gene (plus 70 bp upstream) is replaced by a chlorampenicol resistance gene with 200 bp upstream region. ..

    Sequencing:

    Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy.
    Article Snippet: AR TIC LE IN PR ES S 129 MATERIALS AND METHODS 130 131 Cell lines 132 HeLa cells (ATCC® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium 133 (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 134 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 135 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% 136 FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher 137 Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37oC and 5% 138 CO2. .. 139 140 Plasmids 141 The coding sequence of BRCA1 was PCR-amplified and cloned into pEGFP-C1 (Clontech) 142 by restriction site digestion (SalI/SacII) and ligation. .. BARD1 and RBBP8 coding 143 sequences were also PCR-amplified and cloned into pmCherry-C1 (Clontech).

    Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy
    Article Snippet: HeLa cells (ATCC ® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37 °C and 5% CO 2 . .. The coding sequence of BRCA1 was PCR-amplified and cloned into pEGFP-C1 (Clontech) by restriction site digestion (SalI/SacII) and ligation. .. BARD1 and RBBP8 coding sequences were also PCR-amplified and cloned into pmCherry-C1 (Clontech).

    Polymerase Chain Reaction:

    Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy.
    Article Snippet: AR TIC LE IN PR ES S 129 MATERIALS AND METHODS 130 131 Cell lines 132 HeLa cells (ATCC® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium 133 (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 134 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 135 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% 136 FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher 137 Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37oC and 5% 138 CO2. .. 139 140 Plasmids 141 The coding sequence of BRCA1 was PCR-amplified and cloned into pEGFP-C1 (Clontech) 142 by restriction site digestion (SalI/SacII) and ligation. .. BARD1 and RBBP8 coding 143 sequences were also PCR-amplified and cloned into pmCherry-C1 (Clontech).

    Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy
    Article Snippet: HeLa cells (ATCC ® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37 °C and 5% CO 2 . .. The coding sequence of BRCA1 was PCR-amplified and cloned into pEGFP-C1 (Clontech) by restriction site digestion (SalI/SacII) and ligation. .. BARD1 and RBBP8 coding sequences were also PCR-amplified and cloned into pmCherry-C1 (Clontech).

    Clone Assay:

    Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy.
    Article Snippet: AR TIC LE IN PR ES S 129 MATERIALS AND METHODS 130 131 Cell lines 132 HeLa cells (ATCC® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium 133 (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 134 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 135 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% 136 FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher 137 Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37oC and 5% 138 CO2. .. 139 140 Plasmids 141 The coding sequence of BRCA1 was PCR-amplified and cloned into pEGFP-C1 (Clontech) 142 by restriction site digestion (SalI/SacII) and ligation. .. BARD1 and RBBP8 coding 143 sequences were also PCR-amplified and cloned into pmCherry-C1 (Clontech).

    Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy
    Article Snippet: HeLa cells (ATCC ® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37 °C and 5% CO 2 . .. The coding sequence of BRCA1 was PCR-amplified and cloned into pEGFP-C1 (Clontech) by restriction site digestion (SalI/SacII) and ligation. .. BARD1 and RBBP8 coding sequences were also PCR-amplified and cloned into pmCherry-C1 (Clontech).

    Article Title: Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation
    Article Snippet: .. To generate the mitochondria targeting GFP-cBAK and mCh-cBAK vectors, the cBAK fragment (5′-TTG CGT AGA GAC CCC ATC CTG ACC GTA ATG GTG ATT TTT GGT GTG GTT CTG TTG GGC CAA TTC GTG GTA CAC AGA TTC TTC AGA TCA TGA-3′) was cloned into the pEGFP-C1 (Clontech) and pmCherry-C1 (Clontech) vectors ( ). ..

    Ligation:

    Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy.
    Article Snippet: AR TIC LE IN PR ES S 129 MATERIALS AND METHODS 130 131 Cell lines 132 HeLa cells (ATCC® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium 133 (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 134 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 135 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% 136 FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher 137 Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37oC and 5% 138 CO2. .. 139 140 Plasmids 141 The coding sequence of BRCA1 was PCR-amplified and cloned into pEGFP-C1 (Clontech) 142 by restriction site digestion (SalI/SacII) and ligation. .. BARD1 and RBBP8 coding 143 sequences were also PCR-amplified and cloned into pmCherry-C1 (Clontech).

    Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy
    Article Snippet: HeLa cells (ATCC ® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37 °C and 5% CO 2 . .. The coding sequence of BRCA1 was PCR-amplified and cloned into pEGFP-C1 (Clontech) by restriction site digestion (SalI/SacII) and ligation. .. BARD1 and RBBP8 coding sequences were also PCR-amplified and cloned into pmCherry-C1 (Clontech).

    Countercurrent Chromatography:

    Article Title: Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation
    Article Snippet: .. To generate the mitochondria targeting GFP-cBAK and mCh-cBAK vectors, the cBAK fragment (5′-TTG CGT AGA GAC CCC ATC CTG ACC GTA ATG GTG ATT TTT GGT GTG GTT CTG TTG GGC CAA TTC GTG GTA CAC AGA TTC TTC AGA TCA TGA-3′) was cloned into the pEGFP-C1 (Clontech) and pmCherry-C1 (Clontech) vectors ( ). ..



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    Image Search Results


    In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

    Journal: bioRxiv

    Article Title: Antidepressants interact with sex steroid receptors and their intracellular signaling components

    doi: 10.64898/2026.03.17.712321

    Figure Lengend Snippet: In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

    Article Snippet: A group of HEK293T cells were transfected with a plasmid encoding ERα (pEGFP-C1-ER alpha, Addgene, Plasmid #28230) using Lipofectamine 2000 (Thermo Fisher Scientific).

    Techniques: In Situ, Transfection, Expressing, Staining